OFFICIAL
REPORT
SIK-Up98-9819 © DANÆG AIS (DK) - Dicam Microweaves ApS
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ConfidentiaI
THE SWEDISH INSTITUTE FOR FOOD AND BIOTECHNOLOCY
Official
Report Up98-9819
Validation of Microwave Pasteurisation for Egg-Trays and
EUR-Pallets Contract work for DANÆG AIS
Emma Shah
April 1999
ConfidentiaI – This document may only be used by Dicam Microwaves
on behalf of DANÆG AIS
Confidential
PROJECT INFORMATION
Start of the project 5 October, 1998
End of the project 16December, 1998
The report was finalised
April, 1999
Checked by
Claes-Göran Andersson, Marianne Hedström, Ulf Rönner
Project leader
Emma Shah
Project group
Claes-Göran Andersson, Ewa Eriksson, Marianne Hedström, Farideh Taherinejad
Distribution list
Per Schyum, Danaeg AIS (2 copies); SIK archive; Claes-Göran Andersson,
SIK;
Emma Shah, SIK.
Key words
Pasteurisation,microwave heating, egg tray, egg carton, BUR-pallet, temperature
validation, microbial validation
© SIK1(13)
SUMMARY
Of environmental and economical reasons egg-trays used for transporting eggs
from the production site to the packing facility have been re-used 2-8 times
before they are discarded. Experience has shown that the egg-trays, produced
from recycled fibers, may carry human and animal pathogens and are therefore
a contamination risk. To maintain the materials and logistics used today it
is therefore necessary to pasteurize the egg-trays.
The validation performed by SIK was comprised of two tasks: validating that
the oven (Fabr. Nr. 81400, Svirco) could achieve a temperature of 90oC on the
surfaces of the pasteurised material, and validating the killing effect of the
process on 106 CFU Serratia marcescens. The pasteurisation process was validated
for egg-trays and BUR-pallets with cardboard sheets.
Serratia marcescens has been used as an indicator organism to avoid work with
actual pathogens as e.g Salmonella. Serratia marcescens is, as Salmonella, a
member of the family Enterobacteriaceae.
The measuring points were evenly distributed in the processed material. In all
37 measuring points for egg-trays, a 40 minute processing time followed by a
45 minute holding time resulted in temperatures well above 90oC, and a killing
effect of 106 CFU Serratia marcescens per measured point. In 37 + 9 measuring
points for BUR-pallets and cardboard sheets, a 65 minute processing time followed
by a 45 minute holding time resulted in temperatures just above 90oC, and a
killing effect of 106 CFU Serratia marcescens per measured point.
The process demands that the steam given off during the process is contained
around the processed material during processing and during the holding time,
otherwise the desired temperatures will not be reached.
© SIK 2(13)
BACKGROUND
Of environmental and economical reasons egg-trays used for transporting eggs
from the production site to the packing facility have been re-used 2-8 times
before they are discarded. Experience has shown that the egg-trays, produced
from recycled fibres, are a contamination risk of human and animal pathogens.
To maintain the materials and logistics used today it is therefore necessary
to pasteurise the egg-trays.
As a process was under development for surface pasteurisation of egg-trays placed
on a BUR-pallet, the idea of pasteurising BUR-pallets was also born. Wooden
BUR-pallets are a known contamination risk as they easily absorb moisture and
therefore promote the growth of bacteria and mould. BUR-pallets are also transported
between different places and can therefore carry an infection between different
locations.
In earlier work at SIK, Contract Report Up 97-9434, the elimination of micro-organisms
on egg-trays and BUR-pallets was studied. Based on this work DANÆG AIS
let build a microwave hot-air oven for batch pasteurisation of eggtrays and
BUR-pallets.
The pasteurisation process comprises a processing time in the oven and a holding
time outside of the oven. It also includes the processed material to be enclosed
in a diffusion proof hood to contain the steam given off by the material during
the process. These procedures have been developed during the development of
the prototype oven. The purpose of the pasteurisation process is to ensure that
eggs are not contaminated by the tray material they are in contact with and
that poultry pathogens are not transferred between production sites, i.e. to
achieve a drastic reduction of vegetative pathogenic bacteria on egg-tray surfaces.
© SIK4(13)
GOAL
The validation performed by SIK was comprised of two tasks: validating that
the oven (Fabr. Nr. 81400, Svirco) could achieve a temperature of 90oC on the
surfaces of the pasteurised material, and validating the killing effect of the
process CFU106 CFU Serratia marcescens.
The temperature measurements were to be repeated ten times, and the microbiological
killing effect was to be studied by five replicates, at the least three The
temperature and microbiology tests were combined.
PLANNING AND IMPLEMENTATION OF THE PROJECT
Pasteurization process
The microwave, hot air oven was equipped with 29.6 kW microwave power and 12
kW hot air power. During processing the oven was operated on full microwave
power, and hot air circulation at 90oC. Also, the heated material was covered
by a diffusion proof hood to contain the steam. The use of a diffusion proof
hood results in the hot air having little or no effect on heating of the material.
The heating of materials with microwaves is dependent on the amount of water
ifl the material, i.e. the moisture content. These trials were performed under
real life conditions with a moisture content for egg cartons between 5 and 15
% and a maximum moisture content for BUR-pallets of 25 %. Especially for BUR-pallets
the moisture content could differ greatly between different pallets or parts
of a pallet, but there were no indications that this affected the temperatures
measured.
© SIK5(13)
CONCLUSIONS
In all 37
measuring points for egg-trays, a 40 minute processing time followed by a 45
minute holding time resulted in temperatures well above 90oC, and a killing
effect of 106 CFU Serratia marcescens per measured point.
In 37 + 9 measuring points for BUR-pallets and cardboard sheets, a 65 minute
processing time followed by a 45 minute holding time resulted in temperatures
just above 90oC, and a killing effect of 106 CFU Serratia marcescens per measured
point.
The results are based on the use of the prototype microwave/hot air oven, Fabr.
Nr.81400 developed by DANÆG, as it was configured during the tests at
SIK.
Also, the process demands that the steam given off during the process is contained
around the processed material during processing and holding time.
© SIK - Danaeg DK - Dicam Microwaves 2003
© SIK 13(13)
What is SIK?
© DANÆG / SIK - THE SWEDISH INSTITUTE FOR FOOD AND BIOTECHNOLOGY